ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF CANIS LUPUS FAMILIARIS AND HUMAN SALIVA AGAINST THE PATHOGENIC BACTERIA AND ANALYSIS OF ANTIMICROBIAL ACTIVITY

Authors

  • Ajay Kumar Sahu Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India
  • Prangya Paramita Dept. of Biotechnology, Sambalpur University, Odisha, India
  • Akash Swain Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India
  • Karthika S Pallath Dept. of Bioscience, Mahatma Gandhi University, India
  • Rupali Sinha Dept. of Botany, Purvanchal University, Uttar Pradesh, India
  • Abhisek Soni Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

DOI:

https://doi.org/10.22270/ajprd.v7i3.474

Keywords:

Canis lupus familiaris saliva, Biochemical characterization, Antimicrobial Activity, Microscopic Analysis, pathogenic bacteria.

Abstract

Saliva is mainly contain protein have an important role biological system of that oral cavity is closely associated with different type of disease, in our research work we took canis lupus familiaris saliva which because the composition of dog salivary proteome and with relationship with human are unclear. Salivary proteome have more than 2353 differentially expressed protein human and dog salivary. Protein related regeneration and healing process as fibroblast growth factor and epidermal growth factors regulated by dog salivary, animal saliva is also known as common source of allergen. The main of this study and research work was to identify the particular species and determine the prevalence of  pathogenic bacteria in the saliva of canis lupus familiaris, the biochemical analysis is used to to identify the particular kind of bacteria was present in dog saliva and how its access to another bacteria through antimicrobial activity test was done.  Canine saliva was tested for bacterial effect against pathogens relevant to the hygienic function of maternal grooming of the mammary. Bactericidal against coagulase positive staphylococcus and pseudomonas, e coli is the cause of highly fatal coliform enteritis of mammal. The oral proteomes of dog and human were appreciably different most probably cellular process, development process, multi cellular process, metabolic process, immune system process, biological adhesion is different according to the their biological appearance. These research work provide new information on canis lupus familiaris  saliva composition  and which type of bacteria is present it, and how will be beneficial for the study of dog saliva in diseased and health condition in future.

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Author Biographies

Ajay Kumar Sahu, Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

Prangya Paramita, Dept. of Biotechnology, Sambalpur University, Odisha, India

Dept. of Biotechnology, Sambalpur University, Odisha, India

Akash Swain, Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

Karthika S Pallath, Dept. of Bioscience, Mahatma Gandhi University, India

Dept. of Bioscience, Mahatma Gandhi University, India

Rupali Sinha, Dept. of Botany, Purvanchal University, Uttar Pradesh, India

Dept. of Botany, Purvanchal University, Uttar Pradesh, India

Abhisek Soni, Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

Dept. of Microbiology, Bangalore University, Bangalore, Karnataka, India

References

1. Bakri MM, evaluation of non invasive diagnostic test for helicobacter infection in symptomatic patient and health, Pakistan journal of physiology, 2015; 8:10-12.
2. Cellini L, Grande R, Artese L, Marzio L. detection of helicobacter in saliva and esophagus, new microbiology, 2010; 33:351-357.
3. Diker KS, Akan M, Celik S, Kabakci N. the prevalence, colonization sites and pathological effect of helicobacter in dog saliva, turkey journal vet animal science , 2002; 26:345-351.
4. Farrugia A, Keyser C, Ludes B. efficiency evaluation of DNA extraction and purification protocol on archival formalin fixed and paraffin embedded tissues, forensic science international journal, 2010; 194(4);24-25.
5. Hongs S, Chung Y, Kang KG, Kim O, comparison of three diagnostic assays for identification of helicobacter spp, in laboratory dogs, lab animal research 2015; 31(2):86-92.
6. Niger R, Burnens A, Goke B, Schmassmann A, diagnosis and identification of helicobacter species by PCR in dog saliva sample, microbiology ecology health disease, 1999; 11: 234-240.
7. Sjodin S, Fredriksson M identification of helicobacter DNA in feline pancreas, liver, stomach and formalin fixed paraffin embedded tissues sample, research vet science, 1991; 28-30.
8. Van den Bulck K, Baele M , Debongine JC, Burette A, Stolte M. identification of non helicobacter organism in saliva sample of human, dogs and cats, 2005, journal of clinical microbiology, issues 2005; 43: 2256-2260.
9. Chiappin S, Antoneli G, Gatti R, Elio F. saliva specimen a new laboratory tools for diagnostic and basis investigation, clinical chim acta, 2007; 383:30-40.
10. Guarente L, Sirtuins , Aging and medicine, new engl journal medical, 2011; 364: 2235-2244.
11. Fitzpatrick J, Lendra RB, Jones CL, methods and device for detection of apo A, apo B and ratio there of saliva, 18 January 2017; 234-456.
12. Dale BA, Fredericks LP, Antimicrobial in oral environment: expression and function in health and disease, current issues molecular biology 2005; 117-119.
13. Akpomie OO, Ukoha P, Umukoro G, Saliva of different dog as antimicrobial agents against microorganism isolated from wounds infection, animal science journal 2011; 2:18-22.
14. Lensen cm, Moons CP, saliva sampling in dogs; How to select the most appropriate procedure for case study. Journal veterinary science 2015; 10:504-512.
15. Takagi K, Ishikura Y, Hiramatsu M, Nakamura K, Degawa M, Development of saliva collection device for use in the fields, clinical chim acta, 2013; 425:81-98.

Published

2019-06-15

How to Cite

Sahu, A. K., Paramita, P., Swain, A., Pallath, K. S., Sinha, R., & Soni, A. (2019). ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF CANIS LUPUS FAMILIARIS AND HUMAN SALIVA AGAINST THE PATHOGENIC BACTERIA AND ANALYSIS OF ANTIMICROBIAL ACTIVITY. Asian Journal of Pharmaceutical Research and Development, 7(3), 54–58. https://doi.org/10.22270/ajprd.v7i3.474